I want to start my experiments in flasks in lab conditions. I want to know what the initial biomass concentration DW/L on day 0 should be in Chlorella vulgaris experiments.
Hi Uday Bhan Singh,
I started a previous experiment with 20 mg/L and ended with 300 mg/L after 10 days using WC medium.
Start with exponentially growing cells (midlog phase) for any physiological analysis or protein profiling. We use 250 x 104 cell count for basic biochemical analysis.
It depends on which parameter you are looking for.
Agree with Arumugam Muthu."It depends on which parameter you are looking for."
Why you determine this parameter in terms of dry weight and not in terms of #cells/L or mL?
Dear @Gustavo A. Gutiérrez, Actually I want to estimate proteins and carbohydrates in terms of %age of dry weight.
I think 0.04 to 0.06 mg L-1. it is dry weight for micro-algae when you want to start new experiment.
Best
@Khalid Abdullah Al-hothaly your concentration (dry weight of biomass) i.e. 0.04 mg L-1 is very very low and I think its not good to start experiment with this concentration. check your details.
@Siva Subramanian Sir, The Limit you suggested is very very low i.e.0.02 mg L-1. Please check again. its not possible to measure to weight it in lab. form small cultures like 10ml
Start with 3*10^6 cells/mL. It should be OD680=0.1-0.15 and according to my calculations, around 10-15 mg/L. I hope to be helpful.
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