13 Questions 22 Answers 0 Followers
Questions related from Lorenza Ferro
Buffer A: 0.3M NaCl, 0.03M sodium citrate pH 7.0 (2xSSC) Buffer B: 0.6M KCl, 75 mM Tris-HCl pH 9.0-9.5 I know experimentally that Buffer B has higher ionic strength as compared to Buffer A, as I...
23 March 2023 2,881 0 View
Hello, I have monitored the growth of some microalgal strains along 13 days. I am using a Cell Coulter counter and I made the growth curves by plotting number of cells vs days or tot. volume vs...
20 February 2017 6,026 7 View
Hello, I usually receive a batch of 5 L of municipal wastewater for algal cultivation. I keep it in a cold room while I am not using. I store it as it is and also in small aliquotes after...
09 October 2016 1,763 9 View
Hello, I am planning to simulate summer outdoor conditions in the lab to check the growth of my algal strains in Northern Sweden during summer season. From weather data, I got an average of 18...
25 April 2016 2,013 6 View
I am running some lab-scale experiments comparing different algal strains in different growth conditions. To measure algal cell number I am using a cell Coulter counter (Multisizer II, Beckman...
19 January 2016 6,268 5 View
I want to extract DNA from a wastewater sample coming from a photobioreactor. This sample is full of algae and other microrganisms and I want to recover as much genetic material as possible. Any...
26 October 2015 7,046 4 View
According to your experience, is BBM a good culture medium to cover the growth of different algal species? And what about a temperature of 25°C and a light intensity of 120 μE m−2 s−1 with 18:4 as...
11 September 2015 4,129 9 View
Hi! Is it possible to amplify one gene starting from a mixture of different DNAs? I am asking because I want to determine the population of algae in some water samples through a metagenomic...
03 September 2015 9,245 5 View
Hi, I am extracting lipids from microalgae with a simplified single-step method, in which I use chloroform:methanol 2:1 as solvent; after adding 0.73% NaCl I have phase separation and I should...
10 August 2015 7,603 10 View
I want to calculate spectrophotometrically the concentration of Chl a and b after extraction with acetone 90%. Can I use the equation from Jeffrey and Humphrey (1975), even if green algae do not...
26 June 2015 9,965 9 View
I want to determine the photosynthetic efficiency of some microalgae cultivation through Quantum Yield measurements. Is it ok to measure the QY with light-adapted samples, or is it better to...
26 May 2015 6,673 13 View
Hello, I am preparing stock solutions of unialgal strains in a liquid medium. Which light and temperature conditions are the best to maintain these cells?
13 May 2015 8,728 4 View
Hello, I want to determine the photosynthetic microbial population in some wastewater samples through a genetical analysis. I have a cell counter that can hopefully help me to distinguish between...
09 May 2015 9,883 4 View
