Dear all,

I am planning an experiment to perform qRT-PCR, I was thinking about seeding 300,000 cells per well in a 6-well plate, leave it 24 hours to adhere, then add treatments for another 48 hours. The goal is to harvest about 2 million cells, but the question is what about treatments‘ group, will they yield the same amount of cells as the control group? Or, more accurately a good amount of mRNA to be reverse transcriped into cDNA?

N.B: Such treatments and their concentrations are based upon and derived from a cell viability assay made in 96-well plates on 7000 cell per well. And concentrations to be used are IC50, quarter of IC50, and twice IC50.

Many thanks in advance and best regards.

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