Mark Ashraf Fahmy

4 Questions 4 Answers 0 Followers

Questions related from Mark Ashraf Fahmy

What is the optimal Seeding density for MDA-MB231 cell lines to perform qRT-PCR?

Dear all, I am planning an experiment to perform qRT-PCR, I was thinking about seeding 300,000 cells per well in a 6-well plate, leave it 24 hours to adhere, then add treatments for another 48...

15 July 2023 1,116 2 View

Intracellular calcium quantification, should I use methylthymol blue or O-cresolphthalein?

I need to quantify intracellular calcium, but do not have fura-2am or different calcium influx assay, so methylthymol blue or O-cresolphthalein are the options at hand, then perform colorimetric...

01 June 2023 5,844 1 View

Why my protein vanishes after dialysis overnight?

I am purifying my L-Asparaginase (with 6 his-tag) using Ni-nta chromatography, and imidazole 500 mM + potassium phosphate 0.1 M as and elution buffer and pH of 7.5, and then I check the activity...

02 May 2023 7,265 11 View

Removing imidazole from a purified protein (using Nickel affinity), through ammonium sulphate?

Dear all, I am trying to get rid of imidazole in a purified protein sample (usually 3ml sample), and concentrate it as well. So, I am thinking of using ammonium sulphate method for such a small...

17 April 2023 6,093 3 View