Mark Ashraf Fahmy

6 Questions 5 Answers 0 Followers

Questions related from Mark Ashraf Fahmy

Combination index using compusyn for drugs with two different units?

Hello all, I am working with different compounds against cancer cell lines, and trying to discover whether they are additive, synergistic or antagonistic through CompuSyn. All my compounds units...

03 November 2023 8,198 0 View

Can I make wound healing assay in a 48-well plate?

Dear all, I think that usually wound healing assay are made in 12- or 24-well plates. However, I need to test different drugs in triplets as single treatments and some of them in...

24 August 2023 1,293 4 View

What is the optimal Seeding density for MDA-MB231 cell lines to perform qRT-PCR?

Dear all, I am planning an experiment to perform qRT-PCR, I was thinking about seeding 300,000 cells per well in a 6-well plate, leave it 24 hours to adhere, then add treatments for another 48...

15 July 2023 1,148 2 View

Intracellular calcium quantification, should I use methylthymol blue or O-cresolphthalein?

I need to quantify intracellular calcium, but do not have fura-2am or different calcium influx assay, so methylthymol blue or O-cresolphthalein are the options at hand, then perform colorimetric...

01 June 2023 5,854 1 View

Why my protein vanishes after dialysis overnight?

I am purifying my L-Asparaginase (with 6 his-tag) using Ni-nta chromatography, and imidazole 500 mM + potassium phosphate 0.1 M as and elution buffer and pH of 7.5, and then I check the activity...

02 May 2023 7,272 11 View

Removing imidazole from a purified protein (using Nickel affinity), through ammonium sulphate?

Dear all, I am trying to get rid of imidazole in a purified protein sample (usually 3ml sample), and concentrate it as well. So, I am thinking of using ammonium sulphate method for such a small...

17 April 2023 6,155 3 View