Is there an alternative, more ecological solvent for shape memory polyurethane other than N, N-dimethylformamide (DMF) ?

As DMF creates problems for environmental aspects, we are looking for an alternative solvent for potential commercial end use applications of SMPU.

25 November 2020 8,728 3 View

Can you help me find the specimen of Anotylus sexualis?

Hi everybody, Does anyone have the specimen/s of Anotylus sexualis (Eppelsheim, 1892) (Staphylinidae: Oxytelinae) on own collection? Or, do you know someone else have the specimen on his/her...

21 December 2019 3,122 3 View

It is possible to solve the viscous sub-layer with ANSYS-CFX for sub-cooled boiling flow?

Hello I am solving sub-cooled boiling flow with CFX. I do not want to use single phase wall function. I want to solve the viscous sub-layer. Is it possible? If so which turbulence model is...

12 February 2019 3,685 3 View

What could be reason that as I increase the mesh, mesh dependency is increasing up to a point and after that point it is decreasing?

I am simulating (CFX) a pipe with a turbulence promoter in it. I am using RNG k-e turbulence model yplus is lower than 1. For mesh independency analysis I am using turbulence kinetic energy as a...

29 January 2019 6,801 8 View

What should I pay attention to mesh when I simulate boiling flow?

I am simulating boiling flow with CFX. I want to make mesh sensivitiy study. The problem is that for coarse mesh the solution is converginig. As I do finer mesh I am getting trouble. It is not...

28 November 2018 3,923 8 View

How can I save multiple planes as pictures along the flow domain?

Hello Everyone I am calculating average void fraction for 100 cross sections along the flow direction with "play session". But I want to create and save these 100 cross sections as images. Can I...

04 September 2018 7,016 5 View

How can I change number of my kernel density estimation values without changing 'std'?

Hello eveyone, I have mean value is 60, and standard deviation is 10. I'm generating 50 and 800 samples to see kernel. From kernel density estimation in Matlab, I'm getting high 'std' like 20ish...

25 June 2017 8,038 1 View

Any advice on graphene growth on Nickel and transferring onto glass substrate?

09 January 2015 298 4 View

Could a dirty quartz tube effect on cvd growth graphene?

I've been working on CVD growth graphene for two years. I growth graphene on copper substrate. I didn't have any problem about producing graphene until nowadays but I've some problem about growth...

23 December 2014 8,281 5 View

What software is being used for retention time prediction?

I am looking for the tools to predict peptide retention time for LC-MS.

10 April 2014 9,604 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View