5 Questions 9 Answers 0 Followers
Questions related from Sibel Ciftci
I have RNA template to which I designed several primers to make short cDNA fragments. The reverse transcriptases that I know have strong displacement activities producing long cDNA fragments. Say...
22 January 2016 3,898 3 View
I have sonicated ChIP DNA which smears btw 200-1000 bp. I read that the amplicon size for qChIP should be btw 200-350 bp; however 50-150 bp is recommended for qPCR. Does anyone has experience or...
17 February 2014 9,539 3 View
I need help to analyze the FACs results, which were performed on B cells to assess the cell survivability. I used PI and 7-ADD dyes and I saw two distinct cell populations in the SSC vs. FS blot....
29 May 2013 4,275 4 View
I have been having troubles with qPCR which frequently give signals in the mock samples. My experimental set up consists of samples that were infected with virus and mock samples which were not...
11 February 2013 1,987 11 View
Working with CRISPR-Cas editing in suspension cells. I need a successful delivery of plasmids to suspension cells using nucleofection method ( Lonza Amaxa 4D-X system). The problem is this...
01 January 1970 1,468 2 View
