I isolated total RNA from plasma samples by manual method (trizol), but the GAPDH gene does not appear in PCR. Has anyone worked before?

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Hongdao Zhang

It should be so. Cell-free RNAs (cfRNA) in plasma exhibit notable differences from RNAs found within cells. Typically, one cannot utilize GAPDH or other common housekeeping genes for expression normalization.

Fausto Sánchez-Muñoz

In general I agree with Hongdao Zhang ...can you provide more information about the yield and the purity of your RNA isolation... Few studies normalize circulating lncRNAs with miR-16 or other snRNAs.