What is the number of nuclei in Hepatocytes of Albino rats?

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What are the most effective remote sensing techniques and algorithms for detecting LULC changes in rapidly urban area?

What are the most effective remote sensing techniques and algorithms for detecting and analyzing land use and land cover (LULC) changes in rapidly urbanizing areas, and how do their accuracies and...

11 July 2024 6,695 1 View

Students are found bad at listening as they do pretty well in speaking tests as compared with listening tests? How to overcome this problem?

My attempt to promote listening was "Using Short-stories". It works fairly but there could be better options. Role of Short Stories in Developing English Listening Skill

09 July 2024 9,078 4 View

Using Homophones & Homographs is a challenging task for students. Can you please add your ideas and experiences to help them?

I have tried to resolve this issue a little bit, but eager to get your insights and thoughts to make my efforts better. Using Homophones and Homographs in ESL Classrooms

04 July 2024 6,742 3 View

Would you please share your thoughts and insights to bring reforms in 'Distance Education System'?

The contribution of "Distance Education" is immense, but still needs a lot of reforms. Universities generally adopt one another ideas and ignore the ideas of world wide scholars, distance...

04 July 2024 2,545 6 View

Language teachers can develop speaking skill through listening practices. Can you please suggest some more ways to promote the same (Speaking Skill)?

"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...

01 July 2024 8,406 5 View

How to develop English listening Skill among Hindi/Urdu Speaking students?

In educational institutions where English is taught as a second language, only 25% of listening happens in English but rather 75% of listening takes place in other language like Hindi/Urdu/Punjabi...

28 June 2024 6,353 4 View

How do we identify plant compounds by using thin layer chromatographic techniques?

How do we compare the Rf values of the spots to known standards or literature values to help identify the compounds. How TLC does allows the identification and analysis of the mixture of...

07 June 2024 1,967 5 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

What is the limit of penetration in carbon steel for eddy current based inspection?

Theoretically, at lower A/C excitation frequencies; say around 100 Hz, penetration of 5-10 mm is achievable in carbon steel (ferromagnetic) material. But, there is literature suggesting that this...

10 March 2024 548 0 View

How to set up a PCR protocol?

I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...

28 February 2024 213 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

What is the anti anaemic potentials of hibiscus sabdariffa on wister rats?

anti anaemic potentials if hibiscus sabdariffa

01 July 2024 8,429 0 View

What is the best method to measure diameter of new vessels in tissue,e.g. spinal cord?

Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...

01 July 2024 9,744 0 View