What is the number of blood cells (RBC and WBC) in zebrafish, Danio rerio?

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Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Can a private laptop with 16GB RAM and Intel i5-1235U 1.3 GHz processor be used for WRF simulation with a 12*12 km domain?

I want to simulate WRF for a 12*12 km domain size. I want to run LSM and BSM models with a 5 minutes time interval. My laptop specification is 16GB RAM and Intel i5-1235U 1.3 GHz processor. Can I...

13 July 2024 2,037 0 View

Can zinc oxide (ZnO) effectively purify water, and what are the key mechanisms that make it suitable for water treatment?

I am interested in understanding the effectiveness of zinc oxide (ZnO) for water purification or treatment. I would like to know about its specific mechanisms and applications in addressing water...

14 June 2024 5,389 1 View

How can I define the failure of the beam connector as a discrete fastener?

I am using the discrete fastener method for an LSF frame, and I want to consider the shear failure of rivets in my simulation. However, when I define lower and upper bounds for shear force, Abaqus...

06 June 2024 9,241 0 View

Alginate Gelatin Chitosan hydrogel??

How can prepare Alginate Gelatin Chitosan hydrogel?

05 May 2024 6,229 2 View

Tekla to ABAQUS?

Hello dears I want to import an LSF model from Tekla to Abaqus. Which type of file should the Tekla model be exported as?

30 April 2024 7,978 2 View

Does Gibbs 2 take into account both lattice and electronic contributions to the specific heat Cp ?

23 April 2024 2,280 0 View

Who is interested in participating in a survey regarding the impact of AI on workforce displacement in the logistics industry?

Hi everyone, I'm conducting research on the impact of AI on workforce displacement in the logistics industry for my Bachelor's thesis. If you work in logistics and have experience with AI...

07 April 2024 4,394 1 View

Survey question related to the "AI effect on workforce displacement in logistics industry"?

Hi everyone, Hope you are all in good well and health. I am currently pursuing my degree in Bachelor of Science in Logistics. I am searching for reliable survey questions that I can use in my...

01 April 2024 2,499 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View