I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal values found in human blood, for comparison.
Cited from the article "Determination of trans-arachidonic acid isomers in human blood plasma." published 2004 in Anal. Biochem.: "The mean concentration of TAA in human plasma was 20.2 ng/ml. The levels of isomers were 12.48 +/- 1.28, 2.75 +/- 0.39, and 4.99 +/- 0.74ng/ml for 5E-AA + 11E-AA, 8E-AA, and 14E-AA, respectively." Values were from 16 adults, eight males and eight females. Good luck!
Article Determination of trans-arachidonic acid isomers in human blood plasma