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Questions related from Roberto Boi
We are preparing a transient KD (at the translational level, qPCR is not applicable) and will use targeted proteomics to define the extent of the KD. The KD will be evaluated at 96 hpf (larva...
26 January 2023 8,800 0 View
Set effects are usually quite imponent and mask sample characteristics when dealing with human samples and TMT-labeled non-targeted proteomics. What is in your view the best approach to preserve...
10 November 2022 8,739 2 View
The question says it all. I need to deposit Lipidomics data for manuscript submission and subsequent data sharing.
07 December 2021 3,184 2 View
To use an inhibitor, prepared and sold in a methyl acetate solution, I need to evaporate the aforementioned solvent and exchanging to ethanol or DMSO. I will do that with a gentle nitrogen stream....
02 March 2020 7,149 3 View
I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...
09 January 2020 3,731 3 View
I am preparing a sample for MS analysis from IP. I am going to use TEAB 50 mM as washing buffer. The sample will be then run on a gel and the bands cut out. Given that the pH of such buffer is...
06 May 2019 2,812 0 View
I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...
11 December 2018 9,101 2 View
11 December 2018 8,403 1 View
It is probably one of the most recurrent questions when it comes to WB. Some argue that using GAPDH or other proteins as normalization (HKP) factors is the way. Nevertheless, validation is almost...
01 January 1970 1,977 2 View
