Can anyone know this fungus species?

Can anyone know this fungus species?

05 June 2018 4,092 3 View

Can anyone know this fungus species?

Can anyone know this fungus species?

05 June 2018 6,624 0 View

Can use a mycotoxin compound as a vaccine to livestock?

I am thinking for use mycotoxin banded with large molecules of protein to use it as a vaccine to livestock animals, I dont know if this will work or not to give the animal resistance for...

01 February 2018 6,913 3 View

Any body can know what kind of mushroom is this?

08 September 2017 3,122 3 View

From were i can get a high producer Aspergillus isolate of aflatoxin?

08 September 2017 9,265 4 View

From were i can get a high producer Aspergillus isolate of aflatoxin?

08 September 2017 316 4 View

It is possible for a partial resistance in barley smut diseases?

I have seen many of partial infected spikes of barley with smut disease on the field, as i know smut diseases is systematic infected the plant, is the any strategist diffidence in the plant can...

07 August 2017 8,775 4 View

Any one know a place can supply a insects for teaching proposer?

11 December 2016 4,949 1 View

Anyone know a free software to do the consensus sequence?

09 October 2016 9,846 5 View

Any one know this kind of mushroom?

07 August 2016 7,134 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View