Dear Shadi Aldaghi

1) The optimal medium choice may depend on the specific research question and experimental conditions. DMEM/F12 is the standard according to me because this combination provides a balanced nutrient source for cell growth. It is a widely used cell culture medium, and while it may not be considered the "best," it is frequently preferred for culturing SH-SY5Y cells, according to many scientific articles.

You may want to refer to the papers attached below.

https://biomescientia.com/index.php/lsmb/article/view/23

https://www.nature.com/articles/s41598-024-55516-5#:~:text=When%20culturing%20SH%2DSY5Y%20cells,at%2037%20%C2%B0C7.

SH-SY5Y cells are relatively robust and can be cultured in various media, but DMEM/F12 is often the go-to choice for researchers due to its established use and compatibility with the cell line's characteristics. Moreover, it is a nutrient-rich medium, providing essential amino acids, vitamins, and other growth factors necessary for SH-SY5Y cell survival and proliferation. While DMEM/F12, in combination with appropriate supplements, provides a reliable platform for studying their behavior and properties, other media like DMEM can also support the growth of SH-SY5Y cells.

2) In tissue culture, ensuring high-quality consumables is crucial for successful growth and development of cells. Consumables like culture media, flasks, and other materials must be free of contaminants and of correct composition to support the specific needs of culture being grown. Compromising consumable quality can lead to issues like contamination, poor cell growth, or even cell death.

I can provide you with some useful tips for finding cost-effective options, which will help.

a. You should obtain quotes from multiple vendors for the same products.

b. If your laboratory's needs allow, you may purchase in larger quantities which will reduce the price per unit.

c. While not always as specialized, generic or house brand consumables can be a more affordable alternative.

d. Contact your Laboratory procurement because they may have established relationships with vendors and can negotiate prices for you.

All the Best!