What is the most sensitive way to measure glucose disappearance from cell culture media?

16 October 2017 0 5K Report

We are looking to determine the most sensitive way to measure glucose disappearance (as a surrogate to glucose uptake) from skeletal muscle cell culture media in the short term (1-2 h).

Badges
Science topic

More Craig Sale's questions See All

TC Contamination - What is this?

HELP! Our lab has recently discovered these dancing blobs in our TC cultures - across cell lines, primary cells and organoids. So far they are not behaving like any bacteria/fungi/yeast we have...

30 October 2023 7,792 2 View

Why is my yeast library transformation 10^5 and not 10^8 cfu/ug dna?

I’m trying to reproduce the library transformation efficiencies seen in this 2010 paper: An improved yeast transformation method for the generation o... in which they claim 1.5 x 10^8 cf/ug DNA....

26 July 2023 4,152 3 View

Stem cell therapies for IBD?

Is anyone familiar with publications that outline the success rate and/or effectiveness of stem cell therapies for treating Irritable Bowel Disease (IBD)/Crohn’s Disease?

21 April 2023 1,989 0 View

Can you be our speaker?

Negros Oriental State University, in the Philippines, will conduct an online seminar about "Embedding Gender Issues in Research" on April 14, 2023 from 1:00 PM to 5:00 PM Philippine Time. We need...

19 March 2023 9,477 0 View

Who are willing to speak online for free on embedding gender issues in research?

Negros Oriental State University, in the Philippines, will conduct an online seminar about "Embedding Gender Issues in Research" on April 14, 2023 from 1:00 PM to 5:00 PM Philippine Time. We need...

17 March 2023 1,003 4 View

Methods of creating rod-like micelles with controlled aspect ratios and volume fractions?

It appears that many surfactants will create rod-like micelles under various conditions but I can't seem to find a consensus as to how to smoothly control the shape of these micelles as well as...

07 March 2023 1,248 5 View

Can anyone send me list of affordable publication charges social science journals which are Scopus indexed?

I need a list of affordable journals in the social sciences which are Scopus Indexed. Thank you very much.

23 February 2023 3,430 4 View

What's best tool for rating quality of articles of different types for scoping review?

A colleague and I are independently working on a scoping review on effectiveness of a physiotherapy intervention. One question of the review is what is the spectrum of current levels and quality...

22 February 2023 2,012 2 View

How can I turn a quantitative survey into qualitative interview questions?

I am using self-determination theory as the theoretical foundation for my study of job satisfaction. I have found great instruments used to measure these concepts quantitatively. Does anyone have...

09 August 2022 232 3 View

Framework for Professional Awards Program Evaluation?

Developing a study for a professional technical organization to explore gaps in its awards program. Populations of interest: its members, interest groups associated with organization, as well as...

09 May 2022 704 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View