Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Why can`t I isolate RNA from Extracellular vesicles?

Hello, I have been trying to isolate RNA from EVs (from MSCs) for a while. I isolate the EVs using SEC. Then I use the "Total Exosome RNA and Protein Isolation Kit" from Invitrogen. When I...

21 October 2020 8,559 4 View

How to directly use a directory of .h5 files as the training set in Python without appending them to a list?

I am trying to append .h5 files saved in a directory for my training set using Python, but I face memory error as the Data set is very large. The following code is what I used for...

23 April 2020 9,649 2 View

To record trajectory tracking performance of a controller, is it resonable to calculate the mean_square_error or the normalized-measn-square-error?

I some papers, the mean square error is considered. In some other, the mse is normalized by dividing the error by the (total sampling instants x the total length of the reference...

25 February 2020 784 4 View

How the nonholonomy of mobile robots allow control of the system with less control inputs?

In "Simultaneous Stabilization and Tracking of Nonholonomic Mobile Robots: A Lyapunov-Based Approach", the authors stated that the nonholonomy of mobile robots allows control of the system with...

27 May 2019 7,721 4 View

How to interpret DMA?

18 May 2019 631 6 View

What are the most reliable tools or softwares for designing of the CRISPR gRNAs?

Dear all, Which tool is more reliable? CHOP CHOP or CRISPRdirect? I need some information about other tools and softwares. Thanks a lot

08 May 2019 10,004 2 View

If you could recommend one significant change to make Higher education more affordable without compromising its worth, what would it be?

Higher Education cost is increasing and students encounter some challenges to afford it.

21 March 2019 7,173 6 View

How to match reflection in both visible and infrared?

I have a thin film of a fluorescent material and want to make a reference sample with similar reflection in both visible and infrared to do the comparison. How can I make the reference sample?

26 February 2019 6,857 3 View

Is it true, that herbal remedies have role in management of chronic diseases like hypertension , diabetes and renal diseases ?

in our society a lot of people with chronic diseased use herbal medicine in the course of treatment

13 November 2018 8,285 11 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View