How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

ASTM or ISO standard test for duplex coatings?

Dear all, I would like to conduct scratch and linear reciprocating tribo-test on my magensium alloy, which has a plasma electrolytic oxide (PEO) layer of about 20 um and 10 - 20 um of Parylene...

25 January 2021 9,709 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View

Best Way to Preserve Nitrifying Bacteria in Liquid Culture?

I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...

18 December 2020 5,411 4 View

How to get dry petri dishes in glass after wet sterilization?

Hi, I warping my plates with paper, later put them in the autoclave (use wet sterilization cycle) but still some plates are get wet after ends the cycle. How to get dry the petri dishes? and if...

13 December 2020 8,062 4 View

Nitrate , nitrite , ammonium test can be alternative of total nitrogen ?

I have ion chromatgrahy by which different type of cation and anion can be determined . kjeldal nitrogen arrangement is also available but it is not convenient to me rather than ion...

10 December 2020 1,083 5 View

No title

I am a PhD student, and the doctor asked to do a lecture on ISO 9000, 14000, 17000, 118000,22000 18091,27000 and I would like to prepare the lecture. Is it possible to help me with these...

15 November 2020 8,068 3 View

What for is saturrated sodium chloride solution in ISO 12966-2 ?

Hi, everyone. Our lab is going to use ISO 12966-2 and 12966-4 and there is one moment in sample preparation (rapid transmethylation method under alkali-catalysed conditions) that I can't...

15 September 2020 3,229 4 View

How to remove Coffee-Ring effect in microfluidic paper-based to determination of nitrite?

Hello all together, when I study microfluidic paper-based to detemination of nitrite, coffer-ring effect is my problem but I don't know how to do that to stop. I have attached the image...

30 July 2020 3,184 2 View

The ISO 14040 series standards, Life Cycle Assessment, Is it important?

What do you think about the importance and value-add of The ISO 14040 series standards, Life Cycle Assessment? In general and especially in petrochemical industry.

04 June 2020 3,580 10 View

Relationship between STEP AP209 and CGNS?

Could you please share your knowledge/experience about the relationship between STEP AP209 (ISO 10303: AP209) and CGNS: - Does AP209 covers/supports CGNS 100%? - Where can I read more about the...

22 March 2020 6,625 4 View