I have already gone through that technique. There its mentioned that specimen should be kept for several days for air dry. But in GSI one or maximum two hours we kept for drying.
In our institute, the technician always said that drying is sufficient and thats why specimen has been charged for quite a long time and consequently image quality was not as good as GSI.
Thank you so much Tejabhiram Yadavalli. I did not try ESEM technique yet. for wet specimen it is suitable.
But we have to coat the specimen to make it non conductive, because specimen is biological, i.e. made up of organic matter, so it remains conductive. How is it possible to take picture without coating?
You can search for the paper 'On the preparation of specimens for scanning electron microscopy and a simple technique for plate making, using a black background' by Whittaker and Hodgkinson.