My problem is that i'm trying to precipitate RNA with a Ethanol RNA Precipitation protocol and the yield is bad (almost half of initial concentration and half 260/280 purity). My initial volume is 30 uL and 70 ng/ul of concentration approximately and the precipitation gives me worst concentration and purity than the initial one. Is there a minimum volume for RNA precipitation ? because i'm using the entire 30 uL for the precipitation protocol.
Thanks
I would recommend using the "RNA Clean and Concentrator" kit by Zymo. You can collect several ug of high purity RNA in as little as 6 uL of water. It has never failed me.
Best of luck,
https://www.zymoresearch.com/rna/rna-clean-up/rna-clean-up-concentration/rna-clean-concentrator-5
hi see this link can help you
https://www.thermofisher.com/us/en/home/references/.
Hi,
Your elution volume is probably too high. If your column is not so broad like in the case of Zymo kits, you can try a lesser volume of RNAse free water for a better yield. Also make sure you get rid of ethanol completely before eluting. However, you can also try magnetic bead methods.
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