It's never used... Then the bodies fall in, right?
People prefer static models, without using G!
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
Hello everyone, I'm currently working on calculating the relative levels of mtDNA using qPCR by comparing the Ct values of a mitochondrial gene (nd1) and a nuclear gene. According to my...
16 July 2024 5,508 0 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
One plant was closed it is uncertain what would be the cost of maintaining the infraestructure as industrial heritage. Any idea?
09 July 2024 7,969 0 View
Please can someone share the list of journals with impact factor published in 2024?
27 June 2024 2,927 0 View
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19 June 2024 2,125 3 View
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
We have no access of pressure plate apparatus
22 July 2024 3,488 2 View
Hello, I recently started working in a soil testing lab and am in charge of operating a new piece of equipment in the lab. We are using 4g soil to 40ml extraction reagent, shaking the mixture,...
26 June 2024 1,265 3 View
Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...
25 June 2024 4,602 0 View
I use a DISCO DAD323 dicing machine to cut my sample,which has 1-2 micrometers SIO2 on its top.However,the SIO2 is broken and fall apart everytime.I tried different cutting speed but didn't work....
20 June 2024 1,131 0 View
Dear colleagues, does anyone know of a suitable alternative for Centrifuge software (calculating settling time for grain size separation by centrifugation), preferably one running on Windows 10 or...
17 June 2024 7,085 0 View
Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...
12 June 2024 9,737 6 View
I am researching on azo dye degradation by bacteria. To analyze FTIR, how should I prepare my samples? Do I need to centrifuge, add anything or subtract anything? I am using minimal salt medium...
01 June 2024 3,846 1 View
