20 October 2019 1 2K Report

Melanin concentrations for optimal PCR result

More Andrea Anshy Jácome's questions See All
How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

What keeps journals from switching to a "publish, then review" model of publishing?

A few scientific journals have started implementing a "publish, then review" model of publishing. It is explained well in this article: Implementing a "publish, then review" model of...

13 January 2021 5,449 2 View

Is there any project for a new translation of Freud's 1895 Entwurf einer Psychologie into English?

I'm far from being a Freud expert, but, my feeling is the 1950 translation by J. Strachey is in many ways outdated, when not misleading (starting form the very title of the essay, the awful choice...

10 December 2020 7,005 2 View

What is the main difference of diluting HCL in methanol as opposed to water?

I am hoping to determine the mass per unit area (as per DIN 50938) of a black oxide coating. I use water to achieve the required 1mol/l hydrochloric acidic solution, but a fellow lab had used...

06 December 2020 4,806 1 View

Linear Mixed Models for repeated measures - how can I restructure my data set when some variables are only collected at baseline?

Hello, I plan to perform a linear mixed model analysis to look at change in cognitive function over 5 follow up waves. I am interested in looking at how this change is influenced by dietary and...

27 November 2020 5,087 3 View

Mendeley profiles will be removed in December. What will be of the reader counts from Mendeley as early impact predictors?

Elsevier announced that Mendeley profiles will be discontinued from December. There is a study (Mendeley readership counts: An investigation of temporal and... ) that confirms the value of...

01 November 2020 4,832 4 View

Assessing MCMC convergence by matrix-parameter?

Hello everyone, I need to assess whether a MCMC chain (implemented in BayPass 2.2) has reached convergence and, as far as I know, in order to check it, I must look at the posterior distribution of...

27 October 2020 6,170 3 View

Searching for reliable antibodies for the adrenergic receptors alpha1D and alpha2A for western blotting?

Dear all, I am looking for reliable antibodies for the adrenergic receptors alpha1D and alpha2A, to use in Western Blotting on human skeletal muscle tissue. Anyone with experience with good...

25 October 2020 9,421 2 View

What is the best way to start and develop a scientific collaboration?

What advice would you give to a young researcher who has to develop his/her ability to produce collaborative scientific work?

22 October 2020 5,934 8 View

Temporal noise analysis CMOS APS?

I was reading the paper "Analysis of Temporal Noise in CMOS Photodiode Active Pixel Sensor" but I am a bit puzzled about noise calculation during integration. In particular, can someone explain...

15 October 2020 9,597 3 View

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dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

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