What is the minimum amount of seawater necessary to obtain RNA signal?

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In a OLS regression, how can I perform an interaction when one of the terms follows a quadratic function?

Hi everybody, One of the predictors (A) of my dependent variable clearly follows a quadratic function (A and A squared both significant). I want to see how this predictor interacts with another...

18 February 2021 4,088 7 View

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Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...

18 January 2021 4,487 4 View

What RNase-free solution can I use to transport fresh mice brains?

I will be having freshly isolated mice brains shipped. However, these brains will be eventually used for RNA isolation and immunofluorescence staining, so it is critical that I have them delivered...

07 October 2020 1,343 3 View

What is the recommended maximum length of listening tasks for primary school children?

I would like to know if there are studies that investigated how long primary-school children are able to concentrate on a listening task. Are there official recommendations for a maximum task length?

20 September 2020 1,934 7 View

When does speaker normalization develop in children?

I am on the research for studies that investigate speaker normalization in children. For example, I wonder whether children around the age of six years can already normalize acoustic differences...

16 September 2020 9,907 7 View

How can I prevent Ngn2 neuron-astrocyte co-cultures from peel off the coverslip?

Hello everyone, I've been co-culturing hiPSC-derived Ngn2 neurons with human fetal astrocytes, and at the time of grabbing the coverslip from the plate (24 wp) the cells peel off the coverslip. I...

03 September 2020 2,947 1 View

How can I get individual rarefaction on PAST4 (Paleontological Statistics software)?

Hi, I'm currently working with PAST 4.01 and I have my data organized on a table of 4 columns (treatments) and 45 rows (different bacteria genders) with the amount of genders found in every...

31 March 2020 1,914 1 View

Benjamini- Hochberg correction, which p value use?

We have carried out 6 repeated measures ANOVAS: 2 (group) X 4 (image categories: pleasant, unpleasant, food and neutral) for 3 physiological measures (blink, corrugator and zygomatic) and 3...

20 November 2019 3,451 3 View

Is anyone interested in evaluating the antibacterial or antifungal activities, or the toxicity of their new nanomaterials or nanoparticles?

In the Nanobiology Laboratory we are looking for international collaborators interested in evaluating the effect of the nanomaterials synthesized by them, in different model organisms, prokaryotes...

06 November 2018 1,854 7 View

How can I teach astronomy in middle school?

Sometimes astronomy is a lot like a hard subject to teach about in school, but it's also a very important topic to learn

20 February 2018 3,472 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View