For light microscopy in plants I found this website. It is in German, but comprises a big amount of stains with detailled protocols.

http://www.aeisner.de/index.html

I am no botanist, but would try a trichrome stain like the one-step trichrom Gomori. The higher protein content in the nucleoli is highlighted by acid dyes, chromatin is stained by weigert's hematoxylin.

Thank you dr. Lang your suggestions would be of great help for me. But the problem is the site is in German which I am unable to read. Can you provide me any suitable paper in English giving protocol on plant nucelolus staining.

Thank you dr. sugali. let me try the methods. But my problem is not solved. Can you provide me any good paper giving a detailed account of plant nucelolus staining from root tip cells of Allium cepa or Lathyrus sativus.

I try to translate the Gomori method, described in this website:

Work with paraffin-sections.

1. Deparaffinize the sections in xylen or equal, rehydrate until water

2. Trichrom working solution for one hour or longer.

3. rinse in 100% isopropanol (sometimes 70% with few drops acetic acid works better)

4. dehydrate, xylen and mounting

staining solution:  0.6g Chromotrop 2R (C.I.16570), 0.3g Fast Green FCF (C.I.42053) and 0.7g phosphotungstic acid solve in 100ml deion. water.

fixation in chromatic acid containing medium is preferred.

result:nuclei darkred, Cytoplasma lightrot, Cellulose green, wood red, Cutin yellowish. 

hope this helps, Gudrun

Not knowing about your Portefeuille/portfolio of (staining) methods you could perform in your lab in India. You are scratching a bit beyond the practicable limits of "simple AND fast" staining methods when it comes to localize / find [morphological] evidence for effects of heavy metals (may we ask honestly which one?)  in such small structures/organelles like nucleolus.

My understanding is that nucleolus / nucleolus organizing region or AgNOR  (cit. citation End).       *)  Transmission EM, which in my honest opinion could be also a solution to be considered for your task.

It might be that one can stain fast and simple (as you wished) but without any reliable and / or specific solution of your problem in terms of localization, morphology etc.

One also could try the Palette of specific AMG (Autometallography) for most heavy metals in human/animal tissues and for sure also in plant tissues as introduced / made fashionable by Gorm DANSCHER, who is on ResearchGate, vide: https://www.researchgate.net/profile/Gorm_Danscher . He is for sure willing to help or guide you if you write to him via ResearchGate Messaging (but see also at least the titles his tremendous number of Heavy metal  -  AMG specific publications

Search Google (if this is possible for you) at least for

| plant nucleolus AND nucleolus histolog* stain*AND root tip cells of Allium cepa AND OR Lathyrus sativus |   and you should find 80+ results, most of which might be of value to you and the Task you want to perform. Out of all results for convenience:

Cd++: http://www.jesc.ac.cn/jesc_cn/ch/reader/create_pdf.aspx?file_no=19940314,

Al++: http://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-225,

Pb++: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159802/

I really hope you'll find this helpful anyway, regards and best wishes, WM.

Dear Prof. Muss,

Thank you for your detailed note related to nucleolar staining. I am trying to see the nucleolar mophology and counting after treatment of lathyrus sativus roots with Pb and Cr and trying to see the potency of the metals in nucleolar disruption in comparison to control by simple staining methods. Since lathyrus roots are rich in oils  I asked for a simple and fast staining method to visualize the nucleolar assembly  before and after treatments. I am again thankful to you for your valuable suggestions.

Dear Dipan (you must omit "Prof" in front of my name!), you are welcome.

It might be that I missed your task for simple and fast "over view staining" of affected Nucleoli... so perhaps these hints on literature - but mostly in/on human/animal material - (eventually) could help (NB: all might depend a bit whether you fix and if which Fixative you'll use):

Really old ones, perhaps very simple and fast:

Theoretical hints: SEMMENS (1943), Nucleolar stain and nucleal reaction: http://www.jstor.org/stable/2472153?seq=1#page_scan_tab_contents, (unfortunately only one page) 

AYRES (1947) A method of staining Nucleoli of cells in fresh benign and malignant Tissues (Using AZURE C stain - today one could think of trying a Giemsa stain with animal/human tissues), @http://cancerres.aacrjournals.org/content/canres/8/8/352.full.pdf

HORN  EC, WARD  CL (1957): The localization of basic proteins in the nuclei of larval drosophila salivary glands (Feulgen reaction for DNA localization, combined with

the fast-green-staining method of Alfert and Geschwind ), @ http://www.pnas.org/content/43/8/776

On the cytochemical demonstration of basic proteins in the cell nucleus, including the nucleolus. Jordanov J., Acta Histochem. 1976;55(2):245-54. from: http://www.ncbi.nlm.nih.gov/pubmed/61691

Stain Technol. 1981 Jul;56(4):215-9.Mouriquand J, Mouriquand C, Petitpas E, Louis J, Mermet MA.: Differential nucleolar staining affinity with a modified Papanicolaou staining procedure (as of: http://www.ncbi.nlm.nih.gov/pubmed/6171053 ). Refers to

Peter J Shaw, 2005: NUCLEOLUS [ENCYCLOPEDIA OF LIFE SCIENCES]  @ https://www.jic.ac.uk/staff/peter-shaw/pdfs/Shaw%20ELS%2005.pdf

(Specific) plant stains see:  Safranin O / Fast Green, Alcian Blue  http://microscopy.berkeley.edu/Resources/instruction/staining.htm

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100016

https://www.nottingham.ac.uk/bioenergy/documents/plant-material-testing-activity.pdf

(admitting not to have stained plants either for nuclear or nucleolar proteins/material) 

Best wishes and regards, Wolfgang

According to the excellent answers of Gudrun and Salamma and Wolfgang, your  posted 3  answers show that you are not familiar with internet searches concerning your aim. WHY??

At first please start GOOGLE SCHOLAR   and NOT   GOOGLE.

give in the mask : " Lathyrus sativus and heavy metals" p.e..

and you earn ca. 1390 hits.

https://scholar.google.de/scholar?q=lathyrus+sativus+and+heavy+metals&btnG=&hl=de&as_sdt=0%2C5   and

Lead accumulation in the roots of grass pea (Lathyrus sativus L.): a novel plant for phytoremediation systems?

J Brunet, A Repellin, G Varrault, N Terryn… - Comptes Rendus …, 2008 - Elsevier

... by plants has emerged as a promising, cost-effective alternative to reduce heavy metal levels

in ... identification of plant species able to: (i) tolerate large quantities of heavy metals in the ... Grass

pea (Lathyrus sativus L.) is a ubiquitous annual leguminous crop with such an amazing ...

As assistant professor,, you posted a vague and obscure and obsolete question! WHY???

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What is the most rapid method to stain nucleolus in plant cells, e.g. Allium cepa or lathyrus sativus?

you cannot compare the two plants Allium Cepa and lathyrus sativus. First mistake. The uptake of metals occurs  must be given for your project.

I am trying to see the effects of few heavy metals on lathyrus sativus. I would like to see the effects of these heavy metals on the chromosome vis a vis on nucleolus also

Do you want to stain at first VITAL or do you want to fix the plant cells??

For vital staining please try to obtain  the book via your library (see jpg files 1 and 2)

For fixed cells , please give infos about your preparation of cell smears prior your "rapid staining"

Please give infos about your procedure for your plant chromosome preparations prior to stain. You need a fluorochrome for them.

What a kind of microscope e.g. fluorescence microscope are you using?

. How can I go for nucleolus staining using very simple methods?

As assistant professor, it seems to you perhaps will  work e.g. stain your plant cells or chromosomes like a student and NOT like a SCIENTIST!

In order to resume, the very simple methods are depending of VITAL STAINING or FIXED SMEARS or FIXED  SMEARS for FEULGEN REACTION p.e.

The methods are not simple.

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Please give us infos.

JRG

I send you the cover of a booklet of my co-author (deceased/ W. Kleinwächter). It  doesn 't concern  plant cells but human cells and tumor cells and it gives a lot of hints concerning the basic staining of cell nuclei.

Try to obtain it via uni library.

JRG

Thank you Prof. Jean for your updated feedback, I would definitely look into it.