I am trying to investigate and monitor VSMC migration in mouse model, what is the best way?
The classic paper for ascertaining SMC in vivo migration was published in 1986 (in rats - but the principle is the same). Clowes and Clowes, Circ Res 58: 839-845, 1986. This involves the use of tritiated thymidine, but can be updated for BrdU etc.
One way I read in a paper is you physically scrape cells off a tissue culture plate (like with a blade or something) and observe rate of migration into the portion from where the cells were scraped off.
The classic paper for ascertaining SMC in vivo migration was published in 1986 (in rats - but the principle is the same). Clowes and Clowes, Circ Res 58: 839-845, 1986. This involves the use of tritiated thymidine, but can be updated for BrdU etc.
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