What kind of optimization is done through genetic algorithm in architecture?

Difficulties and types of optimization

11 May 2020 6,177 1 View

Which animal model (rat or mice ) will be appropriate for study of wound healing?

I am to study the wound healing of infected wound in animal model. However I am not sure about the animal model to be used. Whether swiss albino mice or Wister albino rats or Sprague Dawley rats...

26 February 2020 2,565 2 View

What is the maximum rpm and minimum temperature that would facilitate biofilm formation invitro?

I have been trying to obtain the biofilm of Staphylococcus aureus on glass coverslip to visualise it under the microscope, however no fruitful result has been so far obtained.

19 December 2018 787 4 View

How important is cultivating purpose at work/school?

Why should we make our work meaningful to the best possible level - even by running the extra mile? The way to motivate ourselves! This can even be applied to our learning as well - learn not...

06 April 2018 9,228 11 View

What is the role of mindfulness in work/business/leadership?

Role of mindfulness at work/businesses/leadership and day-to-day life. It is interesting to see business organisations such as BlackRock (in New York), World Bank, Google, Harvard Business Review...

23 March 2018 8,511 4 View

How would (and should) the notion of leadership change in the era of AI (Artificial Intelligence)?

How would (and should) the notion of leadership change in the era of AI (Artificial Intelligence)? Interesting and insightful! "Certain qualities, such as deep domain expertise, decisiveness,...

18 February 2018 4,075 1 View

Developing Self-Awareness - How Important Is It In Leaning and Development?

Self-awareness - why it is the most important phenomenon in learning and human development? Why it is an essential skill for leaders of all levels. "Leadership competencies require more than...

08 February 2018 3,390 3 View

Can kindness be taught? Why should we do that?

Can kindness be taught? Why should we do it? What is the significance? This is what The New York Times Article had to say about it. " The Kindness Curriculum is part of a growing global movement...

10 January 2018 3,658 17 View

Why have we started emphasising on adequate sleep in the recent past?

13 December 2017 1,683 3 View

What changes are required at all levels of education to face the challenges/impacts of automation?

We are getting to the same point again and again from different directions - what changes need to be done (in fact, a paradigm change) to contemporary education systems (at all levels - primary,...

08 November 2017 4,702 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View