Colchicine does not allow the spindle formation and the chromosomes are restricted at metaphase without separating at anaphase.

Aceto-carmine staining  is used for the demonstration of nucleic acid within the chromosome.

Colchicine, a potent mitotic inhibitor, can induce chromosome doubling in dividing plant cells.

Cochicine is used to arrest the metaphse chromosomes at polar view for karyomorphological studies also

Dear Anjali Shukla

Pls. find the attached files, good luck on your project

https://naldc.nal.usda.gov/naldc/download.xhtml?id=42088&content=PDF

http://www.academicjournals.org/article/article1380537708_Gueye%20and%20Ndir.pdf

http://www.gordon.edu/download/pages/PDF%20-%20Microspore%20culture%20in%20wheat.pdf

http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=3046&context=etd

https://www.researchgate.net/publication/238783696_Colchicine_induced_embryogenesis_and_doubled_haploid_production_in_maize_Zea_mays_L_anther_culture

http://216.236.251.131/download/pages/Flask%20paper-2002.pdf

https://dl.sciencesocieties.org/publications/cs/abstracts/22/6/CS0220061137

Alisher Touraev, Brian P. Forster, S.Mohan Jain. 2008. Advances in Haploid Production in Higher Plants. Springer Science & Business Media,Pp.348. ISBN140208854X, 9781402088544

http://onlinelibrary.wiley.com/doi/10.1111/j.1399-3054.1995.tb00842.x/full

Regards

Prof. Houda Kawas

Article Colchicine induced embryogenesis and doubled haploid product...

Dear Anjali

Colchicine inhibits microtubule polymerization by binding to tubulin, one of the main constituents of microtubules. Availability of tubulin is essential to mitosis, so colchicine effectively functions as a "mitotic poison" or spindle poison.

The mitosis-inhibiting function of colchicine has been of great use in the study of cellular genetics. To see the chromosomes of a cell under a light microscope, it is important that they be viewed near the point in the cell cycle in which they are most dense. This occurs near the middle of mitosis (specifically metaphase), so mitosis must be stopped before it completes.

About aceto-carmine

A new configuration for carmine is proposed, as well as possible changes to carminic acid and carmine molecules as a result of decomposition caused by heating. Each of the major classes of carmine-based stains is described as are possible mechanisms of attachment to specific substrates. Glycogen binds carmine through hydrogen bonding, and it is here that carmine decomposed by heat could have the greatest detrimental impact. Nuclei and chromosomes are stained via coordination bonds, perhaps supplemented by hydrogen bonds. Finally, acidic mucins react ionically with carmine. Specificity in the latter case may be due to unique polymeric carmine molecules that form in the presence of aluminum chloride (Dapson, 2009).

Best wishes

Acetocarmine is a non-specific nuclear stain which simply binds the chromosomes and gives colour to them. However, specific nuclear stain (e.g. feulgen) reacts with chromosomes to give them the colour. Acetocarmine is a dye obtained from insects.

Colchicine is a mitotic poisoner which arrest the spindle action during cell division as a result of which cells become polyploid. Colchicine is an alkaloid obtained from the tubers of plant Colchicum luteum is used to induce polyploidy in plants.