I send a manuscript to a journal. One of the reviewer made the following criticism. But I do not understand.
"The measurement of RANKL is notoriously difficult. Please provide the success rate of the ELISA analysis completed and CVs for the duplicate samples analysed. This is vital for the adequate assessment of the analysis undertaken. "
How can I calculate the success rate of Elisa results? And how can I find CVs values? Please help:)
Success rate is not a general term when talking about ELISAs. It seems to refer to this particular ELISA, which is particularly difficult according to the opinion of the referee. I would suggest you to show as much primary data derived from this experiment as posible to show him/her that your ELISA results are reliable. The term success rate is also not totally clear for me. It could be related to the proportion of samples for which it was posible to measure the RANKL concentration.
If you make several determinations for the same sample, you can calculate the mean value but also the SD. The ratio SD/ mean value (multiplied by 100) is the CV and measures data dispersion. The lower CV values, the more precise your measurement was.
Hi Omer,
As Gertrudis says the term success rate is not usual when daealing with ELISAs. However its is normal to deal with Sensitivity which is equal to TP/ TP+FN , where TP= True Positives and FP=False Positives, Also you can estimate specificity = TN / TN+FP; where TN = True Negatives, and FP = False Positives.
On the other hand if you want to deal with the detection limit, you must calculate the minimun quantity of RANKL which your test is able to differentiate from a blank.
I see the referee is asking by CV, in that case you should provide data about the standard deviation among your replicates in your assay.
Hope to be of help
Regards
Ramón Fernández
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