What is the maximum passage number that can be used for a study of m-RNA expression of cytokine gene using q-PCR?

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Which type of assay can perform for determination of membrane integrity of gram-negative bacteria?

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How to Detached monocyte-derived THP-1 macrophages form 25 cm2 culture flasks?

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How to design primer for Syber green assay for bacterial gene expression analysis by real time PCR?

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Is there any kit available for especially work for c-DNA synthesis from bacterial m-RNA?

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How do I increase the yield of my PCR product?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Issues with Permeabilization during Flow Cytometry?

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Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

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How to address this issue in the analysis of Real-Time PCR results?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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Where to find data on trees in tropical agroforestry?

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Stephen Karori

I used passage 5 and got good mRNA for 454 sequencing

Ashraf Hussain

Thanks Stephen sir, which cell line are you using? This really low passage number but we are try to buy new THP-1 cell line from ATCC. One of agent from ATCC have only 12 passage. How can I get low passage number cell line from ATCC

4TI cell line. ATCC have very good cell lines with very low passage and well characterized cells