How to design primer for Syber green assay for bacterial gene expression analysis by real time PCR?

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Which type of assay can perform for determination of membrane integrity of gram-negative bacteria?

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Is there any kit available for especially work for c-DNA synthesis from bacterial m-RNA?

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What do you consider to be the most relevant elements of EEG for studying cognitive biases?

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Sushant Shengule

Yup, you can work on design primer software. You can also go for gene runner software for primer design. Whenever your design primer for real time work you must choose product size less than 150 bp. Avoid any loop or hairpin like formation in primer sequence itself.

Md Wasim Khan

us eteh prime3 software

http://frodo.wi.mit.edu/