Ashraf Hussain

5 Questions 8 Answers 0 Followers

Questions related from Ashraf Hussain

Which type of assay can perform for determination of membrane integrity of gram-negative bacteria?

10 October 2015 8,983 6 View

What is the maximum passage number that can be used for a study of m-RNA expression of cytokine gene using q-PCR?

I am using THP-1 cell line for a study of some genes il-8, il-1. What is the maximum passage that can be used for this study? Low passage number is good for a study but we don't have a very low...

02 February 2014 2,837 3 View

How to Detached monocyte-derived THP-1 macrophages form 25 cm2 culture flasks?

In our experiment, monocyte-derived THP-1 macrophages was used for infection with pathogenic bacteria and after Transmission electron Microscope Image, the main problem is, how do we detach theses...

07 July 2013 3,668 13 View

How to design primer for Syber green assay for bacterial gene expression analysis by real time PCR?

Any special software design system for syber green assay ? Can I use primer expression software for designing a primer for syber green assay and how can this be done? I am studying bacterial gene...

05 May 2013 1,185 2 View

Is there any kit available for especially work for c-DNA synthesis from bacterial m-RNA?

Poly (A) tail absent in bacterial m-RNA so oligo dT is not suitable for such purpose, random primer may be more suitable for this. . Can I use any kit have random primer? Are any kit available...

04 April 2013 9,268 4 View