Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Post evaluation of close range photogrammetry experiments ?

I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...

13 February 2021 6,410 3 View

How to define two phase change materials with different operating (boussinesq) temperature in cell zone conditions of FLUENT?

Hello, i am trying to simulate two PCMs in a domain. I assigned their material properties in materials tab. However, when i go to cell zone condition, i can only put one value for boussinesq...

04 November 2020 3,943 3 View

Is there a way to implement Kalman filter on three dimensional GPS data to remove noisy data?

I am trying to implement Kalman filter to get rid of multipath error points on GPS data while my data contains Latitude, Longitude, accuracy, altitude, speed and time. I am using python but unable...

01 November 2020 8,837 3 View

How to choose output capacitor for converters connected to a common dc bus of a SST based dc microgrid?

I am trying to integrate the low voltage dc bus of a solid state transformer(SST) with a dc microgrid. My SST and DC microgrid are operating fine individually. But when i try to connect all of...

11 October 2020 2,090 1 View

How can i interface the microgrid with the main utility grid?

The system below is a dc microgrid. During the islanded mode, the battery buck-boost converter regulates the 400V dc across the dc load. While the ac-dc converter(after the transformer) regulates...

09 October 2020 1,563 1 View

Can we use PEG 400/600 in Dissolution medium, at what level of % ?

Surfactant aid in release of poor soluble drugs but in some cases discrimination is compromised. Can we use PEG if it gives better results in terms of release and discrimination power of...

21 September 2020 6,907 4 View

Issue with absorber layer coating using two step coating including spin coating and dip coating?

I made absorver layer with two step coating. 1) First step was with spin coating in which i used PbI2 by making 1M solution of DMF+DMSO I stiired the soultion for 12 hrs then spin coated it on...

14 September 2020 2,052 1 View

What is the best way or source to search for the Innovator of a drug product?

In Pharmaceutical professional fields such as Regulatory Affairs or Product Development it is of much importance to know the innovator of a product for obvious reasons. But it is not a very simple...

26 April 2020 7,475 1 View

NH4:NaOCl mixing ratio of 3:1 is mole ratio or the mass ratio?

18 March 2020 6,847 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View