What is the source of uncertainty? You can confidently employ RNA markers from various brands in separate wells. Each brand typically offers a size chart, allowing you to cross-reference and verify the size of RNA. Alternatively, you can also utilize a DNA marker. Each RNA species possesses a specific size in base pairs (bp) or kilobases (kb), enabling you to interpret your results accurately based on this information.
I don't see the point in using multiple size markers. Size markers are used to estimate the size of your fragment. If the bands of your marker are too close in the region of your fragment, adjust the concentration of your gel. For instance, for PAGE, if the fragment size is in several kilobase pairs, use a low-concentration gel (between 3.5% and 5%). If the fragment size is in a few hundred base pairs, use a more concentrated gel (between 15% and 20%). I think it's more useful to adjust the gel concentration rather than using multiple different markers.
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