i need to find Ic 50 for docetaxel for pc-3 cell lines using MTT which are treated 24 hour
The IC 50 for cancer cell lines is always altered by the number of passages and the rather predictable development of chemoresistance when performing continuous cell treatment over time to produce chemoresistance subline.
Check this article which show IC50s of 698.91 ng/ml (i.e. 865.1 nM) in PC3 docetaxel-resistant sublines (PC3-Rx) developed in the authors’ laboratory and 28.22 ng/ml (34.9 nM) for a nonresistance PC3 within only 10 passages
Also check the link below in which the IC50 was calculated based on screening of 880 cell lines
Bottom line do your own IC50
Article:
Phosphoproteomic Profiling Identifies Focal Adhesion Kinase as a Mediator of Docetaxel Resistance in Castrate-Resistant Prostate Cancer
Ther; 13(1); 190–201. 2013 AACR.
Link:
https://www.cancerrxgene.org/translation/Drug/1007
Maximum IC50 (uM): 1.42 Number of cell lines screened: 880 Minimum IC50 (uM): 0.000133
You visited this page on 8/8/18.
IC50 is 0.598 nM docetaxel for PC3 cell line following 72 h drug exposure.
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