Lusia Chung

4 Questions 2 Answers 0 Followers

Questions related from Lusia Chung

NFkB expression when treated with LPS on microglial cell lines,is it dose-dependent or time-dependent?

im inducing human microglial cell lines with LPS what i am wondering is that whether the activation of NFKb by LPS is dose-dependent or time dependent? because in latest test i made i had induced...

08 August 2018 5,308 3 View

Nuclear and cytoplasmic protein extraction from trizole?

how to extract nuclear and cytoplasmic protein from trizole after RNA extraction? i want to extract nuclear and cytoplasmic faction from sample after RNA extraction using chloroform, iso-propanol...

07 July 2018 7,761 1 View

What is the IC50 of Docetaxel for Pc-3 cell lines ?

i need to find Ic 50 for docetaxel for pc-3 cell lines using MTT which are treated 24 hour

07 July 2016 380 2 View

My control band(non treated pc3 cells) signal exist in the cleavage of Caspase-3 how do i remove this?

i used 12% SDS-Page and used Pc3 cells which are treated however i still can see the signal in the control band(non-treated pc3) in the caspase-3 cleavage any suggestion how to remove this band?

07 July 2016 3,552 7 View