Housekeeping protein for mice myoblast cell lines
I have had good results in all muscle tissue with B-Tubulin.
Thank you Chad.
I think you should also consider actin and GAPDH.
GAPDH
06 July 2015 9,475 4 View
I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...
11 December 2014 2,038 5 View
I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...
11 December 2014 2,155 6 View
I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...
10 November 2014 1,623 16 View
The housekeeping protein for human skeletal myoblasts
10 November 2014 1,800 1 View
i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...
09 October 2014 7,865 8 View
I am currently using skimmed milk for blocking. My antibodies (anti-phospho antibodies against proteins of insulin signalling pathway) are quite expensive. Please, suggest methods for reusing and...
09 October 2014 934 34 View
I am planning to look at the influence of several proteins on insulin dependent phosphorylation of IR and IRS.
08 September 2014 1,783 2 View
08 September 2014 5,838 6 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
