Housekeeping protein for mice myoblast cell lines
I have had good results in all muscle tissue with B-Tubulin.
Thank you Chad.
I think you should also consider actin and GAPDH.
GAPDH
I used Ser 307 IRS antibody at a concentration of 1:1000 and it was purchased from CST as IRS antibody sampler kit.
06 July 2015 9,617 4 View
I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...
11 December 2014 2,126 5 View
I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...
11 December 2014 2,253 6 View
I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...
10 November 2014 1,701 16 View
The housekeeping protein for human skeletal myoblasts
10 November 2014 1,880 1 View
i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...
09 October 2014 7,993 8 View
I am currently using skimmed milk for blocking. My antibodies (anti-phospho antibodies against proteins of insulin signalling pathway) are quite expensive. Please, suggest methods for reusing and...
09 October 2014 1,062 34 View
I am planning to look at the influence of several proteins on insulin dependent phosphorylation of IR and IRS.
08 September 2014 1,872 2 View
I am checking the effect of various proteins on the insulin signalling
08 September 2014 5,927 6 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
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I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
