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Questions related from Revathy Carnagarin
I used Ser 307 IRS antibody at a concentration of 1:1000 and it was purchased from CST as IRS antibody sampler kit.
07 July 2015 9,576 4 View
I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...
12 December 2014 2,096 5 View
I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...
12 December 2014 2,209 6 View
Housekeeping protein for mice myoblast cell lines
11 November 2014 1,040 4 View
I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...
11 November 2014 1,675 16 View
The housekeeping protein for human skeletal myoblasts
11 November 2014 1,850 1 View
i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...
10 October 2014 7,953 8 View
I am currently using skimmed milk for blocking. My antibodies (anti-phospho antibodies against proteins of insulin signalling pathway) are quite expensive. Please, suggest methods for reusing and...
10 October 2014 1,021 34 View
I am planning to look at the influence of several proteins on insulin dependent phosphorylation of IR and IRS.
09 September 2014 1,846 2 View
I am checking the effect of various proteins on the insulin signalling
09 September 2014 5,900 6 View
