Majid Kamli

4 Questions 7 Answers 0 Followers

Questions related from Majid Kamli

Does anyone have experience to Clone oligos into pLKO.1 neo - based lentiviral vector (Addgene) for shRNA Constructs ?

I cloned my interested shRNA sequence in pLKO.1 neo - based lentiviral vector between Agel and ECOR1. After ligation when I did restriction digestion I got three bands with Nco1 and ECOR1...

07 July 2015 7,946 3 View

Can anyone help with why I don’t see a band of my V5 tagged protein in western blot?

I cloned a segment of my gene of interest in PCDNA 3.1. I checked the sequence by sequencing and everything was fine. Then, I transfected my construct in HEK293 cells. After transfection I could...

04 April 2014 3,256 2 View

Can anyone suggest a tool by which I can locate non coding RNA for a particular gene?

03 March 2014 3,181 2 View

Can anyone help with a problem in sequencing after cloning?

I cloned 900 bp insert in Topo Pc DNA 3.1. After transformation I got few positive clones. I checked these clones by colony pcr and I got the expected size by using forward and reverse primer of...

02 February 2014 1,921 5 View