What is the Gibbs free energy of a normal PCR reaction?

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Houda Kawas

Dear Anirudha Sahu

Pls. find the attached files

http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/gibbspon.html

http://dare.uva.nl/document/2/976

Frank H. Stephenson. 2010. Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory Academic Press,Pp.460. ISBN012375691X, 9780123756916

https://www.chem.tamu.edu/class/majors/tutorialnotefiles/gibbs.htm

https://www.researchgate.net/post/Which_is_the_maximum_tolerable_value_of_Gibbs_Free_Energy_for_DNA_template_self_dimer_formation_in_qPCR

https://absynth.issb.genopole.fr/Bioinformatics/tools/PrecisePrimer/doc/DocWebServer-PrecisePrimer.pdf

http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html

http://onlinelibrary.wiley.com/doi/10.1002/bmb.20461/full

https://www.researchgate.net/profile/Chhandak_Basu/publication/50892954_Real-Time_PCR_qPCR_primer_design_using_free_online_software/links/54f49f720cf2f28c136207a9.pdf

https://en.wikipedia.org/wiki/Nucleic_acid_thermodynamics

Hoping this will be helpful

Regards

Prof. Houda Kawas

Article Real-Time PCR (qPCR) primer design using free online software.

Shufeng Zhang

Entropy: A concept that is not a physical quantity

https://www.researchgate.net/publication/230554936_Entropy_A_concept_that_is_not_a_physical_quantity