Anirudha Sahu

15 Questions 17 Answers 0 Followers

Questions related from Anirudha Sahu

How to make working concentration of around 2000 uM of Temozolomide such that it contains DMSO of 0.22 % or less for use in cell culture?

I am working on a drug called Temozolomide in my cell culture experiments. I am having trouble in preparing working concentrations of this drug from 200 to 2000 uM as the drug is soluble in DMSO...

07 July 2019 7,265 3 View

Can an antibody that can be used for Immuno-cytochemistry can also be used for Immuno-fluorescence ?

I am planning to order antibodies for my experiment which can be used for ChIP, Western blot, Immuno-fluorescence (IF) and Immuno-histochemistry. Some suppliers mention their product to be...

02 February 2018 1,708 5 View

Why Salmon or Herring sperm DNA is used for blocking the membrane and target DNA in Southern blotting?

We all know that Herring sperm DNA or Salmon sperm DNA is used for blocking non-specific binding in Southern blotting. My question is what makes them the best choice for blocking non-specific...

01 January 2018 9,104 1 View

What may happen if our antibiotic vial gets contaminated with a little amount of cell culture?

While treating the adherent mammalian cells in a T-25 flask with an antibiotic (just after changing the media) can we use the same tip for adding the same antibiotic to another flask? Is it ok?...

01 January 2018 5,804 7 View

Can higher dosage of antibiotics added to the mammalian cell cultures affect the cells?

We normally add a very low concentration of antibiotics like Penicillin, Streptomycin, Chloramphenicol or Tetracycline or a mixture of them in the culture plate to avoid bacterial growth in the...

01 January 2018 6,546 1 View

Why my lower MW bands are not visible in SDS PAGE?

I am doing a 12% SDS PAGE gel after total plant protein extraction from cotton leaf by TCA/ACETONE precipitation method. I am loading the crude protein without dilution after keeping it for...

04 April 2017 6,887 20 View

What is the maximum size of amplicon can an NEB Taq Polymerase (normal one) amplify?

What I have heard is the normal Taq Polymerase which we commonly use can amplify a fragment upto 1.5 kb. But yesterday someone told me that the normal Taq Pol can amplify a fragment upto 3kb...

09 September 2016 8,990 3 View

What is the Gibbs free energy of a normal PCR reaction?

I want to actually find out whether a PCR reaction is spontaneous or non-spontaneous and if it is spontaneous how far it is spontaneous?

09 September 2016 1,848 2 View

Does Taq Polymerase require ATP for PCR amplification?

I wonder! we never add ATP as a source of energy for the occurrence of a PCR reaction. Of course we add ATP while adding dNTPs but not as a source of energy. Please clarify.

09 September 2016 8,528 1 View

Is it possible that the plant showing positive for a transgene in T2, the offsprings of the plant in T3 show negetive for the transgene?

I did touchdown PCR using gene specific primer targeting lectin gene inserted into the cotton genome by isolating DNA from T2 plants.The PCR showed positive result in one of the event plant. The...

09 September 2016 5,500 5 View

If one of my PCR component is some how contaminated then is it possible that I get bands in some of my blanks and that to faint and not in all blanks?

Actually I was checking whether my Primer stock is contaminated or not. So I kept 6 blanks and one positive. Out of 6 blanks I got faint bands in 2 of the blanks(all components except template)....

04 April 2016 1,580 4 View

What is the purpose of performing a LAMP (Loop Mediated Isothermal Amplification PCR)?

This method of PCR amplification produces large concatamers of multi-looped amplicons those may show prominent visibility on the gel. But how this confers specificity of the amplification. Or if...

04 April 2016 842 4 View

Can any how combs get contaminated with DNA samples to show bands on the gel even when the wells are not loaded?

Is it possible that combs have accumulated DNA samples from previous loadings. Because for me it seems that every time we run a gel we first remove the comb just after casting and then only run...

04 April 2016 5,514 9 View

What are the sources to obtain neo-natal nymphs of cotton aphid (Aphis gossyipi) for conducting in-planta insect bio-assay?

I need to conduct an in planta insect bio-assay against sucking pest, cotton aphid (Aphis gossyipi) in cotton within this week but not getting any source from where I can collect at least 5000 to...

04 April 2016 9,912 8 View

Can EtBr vaporize at 100 degree celsius?

After removing the gel solution from the microwave we keep it for cooling for nearly 10 to 15 minutes so that the temperature reduces to 55-60 degrees and then only add EtBr. Why can't we directly...

03 March 2016 8,035 3 View