What is the genetic aberration in familial, paroxysmal atrial tachycardia, and who is undertaking (gene therapy) research in this area??
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
As a graduate student at the Arizona State University in the Mary Lou Fulton College of Education, I am beginning my Capstone process. In one of our classes, we have been asked to develop a...
15 May 2024 3,514 1 View
Investigate the socio-economic status of women in the Western Cape, including factors such as labour force participation, wage gaps, access to education and healthcare, and representation in...
25 March 2024 7,972 1 View
La participación de la familia en la intervención con los menores infractores es indispensable, mediante el trabajo con esta se puede mejorar en gran cantidad las perspectivas de futuro del menor....
10 March 2024 392 0 View
Con esta cuestión se pretende conocer las bases técnicas y metodológicas para poder ofrecer a los menores una intervención concisa y eficiente. Principalmente, fomentando su autonomía, su...
10 March 2024 168 0 View
Hello colleagues, I am trying to isolate extracellular vesicles, which tend to aggregate. Is there any method to prevent them from aggregating?
04 October 2023 1,024 2 View
Dear all, We are interested in measuring Arc expression in our animals after fear conditioning. But we are not sure whether injecting ketamine or sodium pentobarbital could alter the expression...
25 September 2023 2,803 1 View
Diagnostic validity of optic nerve head colorimetric assessment and optical coherence tomography angiography in patients with glaucoma Article Jul 2020 Carmen Mendez-Hernandez Surina Wang Paula...
20 March 2023 6,862 0 View
Hi everyone, I have recently used an ELISA kit from Elabscience (E-EL-0047), and the results I got are strange. I have used two different kits and in both of them, the standards and the samples'...
09 March 2023 9,607 0 View
Education is very important from the moment we are born, since from a young age we learn from our reference models that these cases are going to be our parents and the people with whom we spend...
22 February 2023 6,956 7 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View