EDTA is a chelating agent used to remove DNAse from the environment after the nuclear membrane has been broken down.

EDTA binds divalent cations such as calcium and magnesium. Since these ions help maintain the integrity of the cell membrane, eliminating them with EDTA destabilizes the membrane. Tris is the main buffering component; its chief role is to maintain the pH of the buffer at a stable point, usually 8.0.

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