Image J software?

Can any body let me know the current source for the download of Image J software.

04 May 2018 6,269 3 View

What is the real meaning of a "predatory journals", is there any need to handle them?

Many discussion are going on on predatory journals (PJ). Based on few high ranked publications, the definition of PJ seems limited. How the predatory publishers be identified and is there any need...

04 May 2017 5,071 27 View

Why superoxide is name so?

Why superoxide anion radical (O2.-) in named so. Is there any specific reason?

31 December 2014 779 2 View

Should review be written by only workers having related research back ground?

In many cases a review is rejected from a journal citing the reason as the author(s) don't have enough research experience or publications in the related field. However, the early career workers...

31 December 2014 1,771 0 View

Why an enzyme shows increased activity with respect to time (h) with unlimited substrate in a controlled system?

07 August 2014 3,100 10 View

Is western blotting a good tool for target protein quantification?

To quantify a protein under experimental conditions, western blot is used as a major tool. The data is then represented as the level of the same protein and then correlated with its functional...

11 December 2013 9,031 17 View

Can Arrhenius Temperature Break Points be calculated from the simple slope between the temperature and the rate of reaction?

I saw in literature that people are considering the rate of reaction in log scale with temperature in 1000/K unit to calculate Arrhenius Temperature Break Points (ABT). However, if ABT is the...

08 September 2013 7,186 0 View

PCR for Annexin II in cancer cell lines - can anyone help?

08 September 2013 5,487 4 View

Why are bacteria not able to develop their own mitochondrial system for the electron transport chain?

It is believed that mitochondria have evolved from bacterial systems. We all knew that mitochondria have a very precise electron transport chain system and have a clear cut pathway to maintain...

11 December 2012 6,846 9 View

What could be the output in science if funding could go to the right person who really intends to do research passionately?

In many cases, funding and publication happens with a non-disclosed compromise between two known persons. In that case, the output of science is reduced a lot. But its unimaginable how much this...

11 December 2012 2,744 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View