During transcription, heterogeneous nuclear (hn) RNA goes through splicing steps to have the copy of required mRNA. Where the introns move then as small piece of poly or oligo oligonucleotides?
Can any body let me know the current source for the download of Image J software.
04 May 2018 6,382 3 View
Many discussion are going on on predatory journals (PJ). Based on few high ranked publications, the definition of PJ seems limited. How the predatory publishers be identified and is there any need...
04 May 2017 5,189 27 View
Why superoxide anion radical (O2.-) in named so. Is there any specific reason?
31 December 2014 906 2 View
In many cases a review is rejected from a journal citing the reason as the author(s) don't have enough research experience or publications in the related field. However, the early career workers...
31 December 2014 1,898 0 View
During standardization of an enzyme to study its activity, it showed a linear trend of increase up to 4 h then it gets stabilized. For the first time I got such an enzyme to have its maximum...
07 August 2014 3,210 10 View
To quantify a protein under experimental conditions, western blot is used as a major tool. The data is then represented as the level of the same protein and then correlated with its functional...
11 December 2013 9,160 17 View
I saw in literature that people are considering the rate of reaction in log scale with temperature in 1000/K unit to calculate Arrhenius Temperature Break Points (ABT). However, if ABT is the...
08 September 2013 7,309 0 View
I used the primers (5′- GATGTGAAACACTTTGCTCCT-3′ (sense) and 5′-ATAGCGACACTTGGATAGGGG-3′ (antisense) cited in different literature's for Annx II to have RT-PCR amplification in cancer cell lines...
08 September 2013 5,605 4 View
It is believed that mitochondria have evolved from bacterial systems. We all knew that mitochondria have a very precise electron transport chain system and have a clear cut pathway to maintain...
11 December 2012 6,967 9 View
In many cases, funding and publication happens with a non-disclosed compromise between two known persons. In that case, the output of science is reduced a lot. But its unimaginable how much this...
11 December 2012 2,845 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View