Image J software? - FAQS.TIPS

More Biswaranjan Paital's questions See All

What is the real meaning of a "predatory journals", is there any need to handle them?

Many discussion are going on on predatory journals (PJ). Based on few high ranked publications, the definition of PJ seems limited. How the predatory publishers be identified and is there any need...

04 May 2017 5,189 27 View

Why superoxide is name so?

Why superoxide anion radical (O2.-) in named so. Is there any specific reason?

31 December 2014 906 2 View

Should review be written by only workers having related research back ground?

In many cases a review is rejected from a journal citing the reason as the author(s) don't have enough research experience or publications in the related field. However, the early career workers...

31 December 2014 1,898 0 View

Why an enzyme shows increased activity with respect to time (h) with unlimited substrate in a controlled system?

During standardization of an enzyme to study its activity, it showed a linear trend of increase up to 4 h then it gets stabilized. For the first time I got such an enzyme to have its maximum...

07 August 2014 3,210 10 View

Is western blotting a good tool for target protein quantification?

To quantify a protein under experimental conditions, western blot is used as a major tool. The data is then represented as the level of the same protein and then correlated with its functional...

11 December 2013 9,160 17 View

Can Arrhenius Temperature Break Points be calculated from the simple slope between the temperature and the rate of reaction?

I saw in literature that people are considering the rate of reaction in log scale with temperature in 1000/K unit to calculate Arrhenius Temperature Break Points (ABT). However, if ABT is the...

08 September 2013 7,309 0 View

PCR for Annexin II in cancer cell lines - can anyone help?

I used the primers (5′- GATGTGAAACACTTTGCTCCT-3′ (sense) and 5′-ATAGCGACACTTGGATAGGGG-3′ (antisense) cited in different literature's for Annx II to have RT-PCR amplification in cancer cell lines...

08 September 2013 5,605 4 View

What is the fate of introns after splicing from hnRNA?

During transcription, heterogeneous nuclear (hn) RNA goes through splicing steps to have the copy of required mRNA. Where the introns move then as small piece of poly or oligo oligonucleotides?

03 April 2013 8,504 0 View

Why are bacteria not able to develop their own mitochondrial system for the electron transport chain?

It is believed that mitochondria have evolved from bacterial systems. We all knew that mitochondria have a very precise electron transport chain system and have a clear cut pathway to maintain...

11 December 2012 6,967 9 View

What could be the output in science if funding could go to the right person who really intends to do research passionately?

In many cases, funding and publication happens with a non-disclosed compromise between two known persons. In that case, the output of science is reduced a lot. But its unimaginable how much this...

11 December 2012 2,845 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,789 4 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Why is there a significant edge deviation in radar point cloud and camera registration?

The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...

23 July 2024 7,479 3 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Which is the best approach for anomaly detection in scanned image data set?

Anomaly detection in scanned image data set

18 July 2024 3,578 3 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Aldo Von Wangenheim

Dear Mr. Paital,

you'll find the source here: https://imagej.net/Source_code. There's also a more documented, older, source code repository here at NIH: https://imagej.nih.gov/ij/developer/source/index.html

Biswaranjan Paital

Thanks Prof. Wangenheim. I tried the same but that link is not working. Any working link? The original version is under repair mode by the website.

Alexander Novoselsky

You can try Fiji (distribution of ImageJ), https://fiji.sc.