Hi Emmanuel

Amplicon size for real good RTPCR need to not exceed 100-150Bp when respecting the delta delta CT protocol.

for miRNA since the targets are really smaller, amplicon size turns around 50Bp.

see this old paper, it can help:

Article Real-time PCR quantification of precursor and mature microRNA

all the best

fred

Which kind of protocol you use for microRNA-cDNA convertion. Like stem-loop method and polyA tailed protocol. I get cDNA amplicon size around 75bp when using polyA tailed method.

and it's perfect as the paper mentioned

fred