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I run qPCR experiment by using sybr green assay. I run 10ul reaction. I get nice melt curves. How ever, the amplification curves are plain not picking up the threshold. Also my endpoint PCR shows...
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Can we measure the expression of cDNA generated by TaqMan advanced miRNA cDNA synthesis kit by sybr green. Is any have used it before?
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I designed Primers for miRNAs using mirPrimer algorithm. However when l analysed them by Oligoanalyzer 3.1, it shows Primer-dimer and secondary structure formation. I request your suggestion...
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How miRNA quality and quantity could be checked after total RNA extraction from serum either by kit method or trizol reagent?
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