I used P58 this time and saw very low entry of InlB coated beads compared to what I usually see. I was wondering if the passage number has any effect on the Met receptor expression that resulted in lower infection during my experiment.
Hi all, Has anyone frozen the murine tissue samples (lung or spleen) in -80 and then later thawed these to go ahead with cell purification (PMNs etc) for RNA extraction? I am wondering if it...
10 November 2018 9,243 3 View
What is the molecular weight of mTOR expressed through pcDNA3/AU1-plasmid containing rat mTOR gene. I am using this plasmid and when I immunoprecipitate myc tagged mTOR with myc antibodies, I am...
07 August 2014 1,633 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
02 March 2021 3,060 3 View
Is there a powerful system for the security of the systems distributed on IoT systems?
02 March 2021 3,858 10 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...
01 March 2021 2,272 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View