Wahyu Dwi Saputra

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Questions related from Wahyu Dwi Saputra

What is the best way for administering anti-inflammasome candidate in cell experiment?Before second inducer (ATP) or simulatenously with ATP?

Dear colleagues, currently I am doing an inflammasome-related experiment using cells. I have a substance as a candidate for anti-inflammasome. However, I am a little bit confused about which step...

15 November 2019 2,571 3 View

What is the effect of single extracellular ATP administration to the physiology of cell culture?

We knew that inflammasome event induced by LPS and extracellular ATP could lead to the cell pyroptosis and death, but what about the effect of only ATP administration to the cell culture? Is it...

20 September 2019 3,775 2 View

Can we use RT PCR to asses inflammasome inhibitory activity ?

Currently, I am studying about the inflammasome activity inhibition using a compound in cultured microglial cell lines. As the common procedure to analyze inflammasome activity is by measuring the...

09 October 2018 1,251 4 View

Do the combination of skim and sucrose cryoprotectant give less protective effect during freeze drying than single of skim or sucrose?

In my previous research, I used skim 10%, sucrose 10% and combination of both skim and sucrose (10%:10%) as cryoprotectant for freeze drying of Lactobacillus plantarum. I found out that the...

21 June 2015 8,515 3 View

Are there any effects of adding cryoprotectant during freeze drying upon adhesive characteristic of probiotic to the human intestine ?

Freeze drying is commonly used by dairy based industry to produce dried culture of probiotics. While freeze dry processing, usually they add cryoprotective agents like skim or sucrose or MSG to...

30 January 2015 852 3 View