My 5XFAD transgenic mice ( Alzheimer's disease) model raised in a 14h/10h light/dark cycle room.In both left-right discrimination T test and radial arm test, both are appetite motivated, the results showed no difference after training. I wonder whether is the longer light cycle affect the behaviour of the mice?
I guess, if they have been raised from birth under 14h/10h cycle, it should not have any effect, which can surmount the genetic effect. At least not on the positive side. Anyway for solid answer, you may have to compare the same transgenic mice raised in 12h cycle. If you find any effect, it may be a great discovery.
I think that you are focusing to strong a variable which alone might not explain the effect you are observing. Every single animal facility is particular: light, food, air humidity, exposure to noise, exact genetic background of the mice you backcross...
Not reproducing already published and accepted results unfortunately happen, therefore running pilots might avoid to spend time and money on paradigm not adaptable to your facility.
To my mind, the most important parameters to control if you are doing cognition and anxiety tests are (please ignore the order): light and noise intensity in the testing room, age and gender of the mice, light/dark phase, training of your personal and handling of the animals (I mean the number of other tests that these mice experienced before going to these tasks).
Good luck to figure out the best conditions to run your project!
are thee any good literature on augmented reality in museums focusing on war museums?
What exactly do you mean by 'appetite motivated', and why was that important to mention? I assume that whatever groups you are testing (experimental vs control) differed during training, but not in post-training. Is that true? I general, I agree with Nicolas Luigi Pascal Casadei.
I think it would be more important to consider the time when you perform the trials than the length of light-dark hours.
You say you did not find any difference: before and after training? Should your transgenic mice have the capacity to learn those tasks?
Here we discussed some of this
Article Behavioural phenotypes in the cuprizone model of central ner...