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Questions related from Nicolas Luigi Pascal Casadei
I would like to use relatively old lysate in an experiment (10 years old lysate). Because some of my antibodies are reconizing full length proteins, but also fragments and oligomers, I want to...
18 January 2015 4,201 9 View
I try to read enzymatic activity in tissue homogenates using fluorescence emission in a microplate reader. I hypothesize that a specific treatment can reduce amount of enzyme in tissue and/or...
07 January 2015 3,280 10 View
In immunohistochemistry, many protocols recommend to block the tissue to analyze prior the first antibody incubation with the serum of the animal in which the secondary antibody is raised...
31 July 2014 8,865 20 View
I noticed that some CO2 suppliers provide different range of puritz (from 99.0% to 99.99%). I was wondering how much CO2 quality may impair or enhance reproducibility of cell culture...
21 July 2014 9,021 9 View
I am designing a chromatin immunoprecipitation experiment to map histone modification potentially present in a mouse model. In order to choose which histomodification I am going to IP, I would...
20 January 2014 9,139 4 View
How should I discard high quantity (around 10 liters) of antibiotic (amoxillin)? I was thinking to warm it up for a while to degrade the molecule but I am not sure it fulfill authorities...
07 February 2013 2,425 2 View
I was wondering what would be the effect of too high a concentration of APS in an acrylamid gel. I am also curious to know how people manage their stock of APS (we dilute APS with water at 10%...
14 January 2013 3,516 12 View
Are antibodies already HRP-conjugated more sensitive than the two steps "antibody + washing + anti species HRP-conjugated"? If yes, kind you suggest any kit to do it?
21 December 2012 9,123 10 View
As previously posted on the forum, we are using an illuminated glass plate to measure gait disturbance in mice. As you can imagine, speed is directly positively correlated to a high number of...
28 September 2012 7,731 10 View
We are transfecting HEK cells using a vector expressing a protein normally located in the nucleus of cells. By mutating the nuclear localization signal of the protein of interest, we would like to...
11 September 2012 9,875 13 View
