12 Questions 101 Answers 0 Followers
Questions related from Nicolas Luigi Pascal Casadei
I would like to use relatively old lysate in an experiment (10 years old lysate). Because some of my antibodies are reconizing full length proteins, but also fragments and oligomers, I want to...
18 January 2015 4,209 9 View
I try to read enzymatic activity in tissue homogenates using fluorescence emission in a microplate reader. I hypothesize that a specific treatment can reduce amount of enzyme in tissue and/or...
07 January 2015 3,292 10 View
In immunohistochemistry, many protocols recommend to block the tissue to analyze prior the first antibody incubation with the serum of the animal in which the secondary antibody is raised...
31 July 2014 8,878 20 View
I noticed that some CO2 suppliers provide different range of puritz (from 99.0% to 99.99%). I was wondering how much CO2 quality may impair or enhance reproducibility of cell culture...
21 July 2014 9,026 9 View
I am designing a chromatin immunoprecipitation experiment to map histone modification potentially present in a mouse model. In order to choose which histomodification I am going to IP, I would...
20 January 2014 9,143 4 View
Has anyone seen any literature on this topic? Has anyone tried to measure the influence of this parameter on the performance of mice in this test?
20 April 2013 7,976 2 View
How should I discard high quantity (around 10 liters) of antibiotic (amoxillin)? I was thinking to warm it up for a while to degrade the molecule but I am not sure it fulfill authorities...
07 February 2013 2,432 2 View
I was wondering what would be the effect of too high a concentration of APS in an acrylamid gel. I am also curious to know how people manage their stock of APS (we dilute APS with water at 10%...
14 January 2013 3,524 12 View
Are antibodies already HRP-conjugated more sensitive than the two steps "antibody + washing + anti species HRP-conjugated"? If yes, kind you suggest any kit to do it?
21 December 2012 9,129 10 View
To test cell cytotoxicity at different time points in the same sample, is Invitrogen’s Alamar Blue (non-toxic) a better alternative to the MTTassay by Sigma/Millipore/R&D Systems/Promega etc? Has...
16 October 2012 7,014 4 View
As previously posted on the forum, we are using an illuminated glass plate to measure gait disturbance in mice. As you can imagine, speed is directly positively correlated to a high number of...
28 September 2012 7,743 10 View
We are transfecting HEK cells using a vector expressing a protein normally located in the nucleus of cells. By mutating the nuclear localization signal of the protein of interest, we would like to...
11 September 2012 9,881 13 View
