what are the consequences if I will either TAE buffer or SDW for dilution of DNA sample ?
What kind of changes can occur by using either one ?
EDTA is a chelating agent which chelates the cations like Mg2+. This thing protects DNA from degradation.
If you want to keep your Stock for longer time then by using the elution buffer (which is typically Tris-EDTA buffer) helps to maintain the pH and the conditions for stabilizing the DNA for a longer time.
on the other hand SDW is also a good options until it is not contaminated. i always use SDW to dilute my DNA stock.
Sheelu is right, TE Buffer is preferred, but you can still use sterile deinoized water for short term.
TE buffer is OK to use, so long as your nucleic acid concentration is high. So for example, if you had enough nucleic acid that you had to dilute it 10x or 100x for use in an enzymatic reaction—TE is fine. But if the concentration is poor, it is better to use nuclease-free water (I buy it commercially—just better that way). The reason is because, if you need to add 30 µl of poorly-concentrated nucleic acid to a 50 µl enzymatic reaction, there will be so much EDTA in there that, not only will it protect the nucleic acid—it will also likely inactivate the enzyme you are using (if it uses divalent cations, and many enzymes do). So, I use TE when I have a large pellet during extraction, and sterile water for when I have a very small (or invisible) pellet.
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