What is the effect of defatting method on the production of chickpea isolate?

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İsmail Emir Akyildiz

What is the extraction method you used or planned to use? Are you extracting only the water-soluble protein content from chickpea flour? Detail about your strategy needed to be known to exclude defatting...

Razie Razavi

Thank you İsmail. I want to use the cold press deffating method using n-hexane or petroleum ether and followed by acidic precipitation. Can I remove the dettating stage? The chickpea protein will be used in bakery products..

Hamioud Aya

i think the defatting process can increase the purity and the yeild of the extracted proteins since lipids can make an obstacle for protein extraction

you can see this article for more info : https://pdf.sciencedirectassets.com/272562/1-s2.0-S0733521023X00046/1-s2.0-S0733521024000341/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEH8aCXVzLWVhc3QtMSJHMEUCIQCG19vYwwRdNxDYUrUc8l6pL%2BnEyZeBxMw1Wd1SUs0rhwIgCe7draGKJ1%2FjOXcT9bAoroTq7BMjYN3YODFzZHY%2Bd1UqvAUI%2BP%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FARAFGgwwNTkwMDM1NDY4NjUiDM7khVO8VPPaIUs3ECqQBfRQtN4TveI8WJ8SWY6AcKLvd6maiJvIC9fF7GZSLeBkxA71I6c%2BxnlFtcXSy9J5Y7m2e52B32aZfdqSGaKbpAewikemNJJYZHQdRQJfNofx%2FgMx%2BKuus6dJ9sf%2FvdAkH14XWUhM47cY2xjZUazWxM6Viad3dgfkibJooqstSq%2F7HZnqtFFsPWhKmx6py%2FcVaFb4NDq4ShfzBhJSIEaFUj37G5fgSW7bE3rKBT%2Bn4ClSQMP7iHfvodaLEDAu4aBEEzXkpwZ%2FTNxtSi5B5GLC%2BgHjklw%2BVcrcvLOxjy4Jfwrh6SXsoE8k%2FxWmyPhp%2BzI%2B81zA9OgsMMxKOCzjQIZMjhiPGSy5gqHrHqBv6dvCAAZ9iWHQRaKtZkiRTA0hj6As2uB9Oxj42Rxs9qTJMj6nC873L8am%2Fh3mgdzRTGZnPKO3NiCSrKOVqd6m3r1JwTnjnjKAUABHHPBJ%2B71DgNsM1oOcvDZ1tDPdLE%2B8QIDZTrk%2Bhyp2msIG03mcdh8HK9qgFq83YrCXMl7awIm7vtucsq8e8iEuiaQDen4qIrZAIa%2BTJ0GCd4mSUqDxeTTIGta0V%2Fh74ZF3QIcX7Z3NXgLDWr4wfiCJQ%2F2IVjuGJ6qN%2BJYn0pwYrYg7%2Fl0kPxiO%2B56j9rX%2FLi3bn1irIxA0hLC13goFGa9JYbMqrbiCd9axmhlvaQC09EI9Lpvm8w8YFw4RmUj64wA%2BEp64dGaX3UBXGwH%2BZBNIU%2FZW%2BrTpE34Ympwi4y4lDAWVefAXvYnloSCakxvIeD7ZWItc01%2F2gzLISxywJAQiiGo19l2evYHYG9wC3k5%2FkMhHOPISHpLNo0jpnBZcWRMN76u2bIXNZvD%2BlpS1o3iiNDSawC4FCWnyPdcIMMm62bIGOrEB25GktOhaF2Y5rCDdLuIUAAQ7J7oOnjl3h%2F%2FNgF%2FTo2Yr0wLe2Ff8L%2BgqhVBMCdQr94f1gMupuN2PrDHM0NtBHGc7MsmuQn7yDk%2Bx3GvCDGydc8WOyI0OOJQyJVu844Rhit9Fs91yEVB4eyqSQcXC2SsGNzfbwQj5daR20ejl%2F18JTnB6pVT1Hy2ERFRSUkM1UVG6sCCHHrOit0OEvM1aVr7j7aWxCWhHKNK5Mfez%2BbGV&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20240528T232044Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY5G32WWGV%2F20240528%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=f6a8b05e305b549dda3dc9f7da61b164306e881165e4d75a932f0f2f2f40bec2&hash=67801474b2e734f5ca0de7eb6f203a398506796d1c45c22db6b8a8d70b513f5e&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0733521024000341&tid=spdf-500cf00b-9cb3-49a9-9322-fb3af51c826d&sid=d19991297bfbb34bf32987e6f8e310a56c52gxrqa&type=client&tsoh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&ua=001d5e590056090e5f&rr=88b1fa1d98943375&cc=dz